Dear colleagues,
How can I convert fertilizer rate from Kg/ha (Field) unit to g/kg of soil (Pots: green house)?
Dear colleagues, I want to use an insoluble potassium product to asses the effect of bacteria solubilizing potassium; Can you suggest a chemical product containing insoluble K! Thank you
10 November 2017 4,467 5 View
Dear colleagues, I find, in most articles, that they use the Aleksandrov medium for screening bacteria solubilizing potassium. But this Medium contains also insoluble phosphate (Ca3 (PO4))2,...
09 October 2017 2,734 0 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...
25 February 2021 8,039 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?
23 February 2021 3,053 7 View
Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...
21 February 2021 2,084 1 View
Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...
21 February 2021 4,388 3 View
What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...
21 February 2021 1,831 4 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View