Alternative method of Permeablization in IL17A assay by flowcytometry?

I am evaluating Th17 cells by staining stimulated cells with IL17A and IFN-Y antibody by flow cytometry. Permeabilization step was done with Cytoperm/cytofix reagents (BD Bisciences) yet and the...

01 December 2020 4,553 1 View

Suggestions how to get Shapefile of India with all 700+ Indian districts?

I need shapefile for all 700+ districts of India. From where I can find the India shapefile with all districts. Also, suggest how to get the latest India shapefile with all state and union...

07 August 2020 3,449 5 View

PSO, ABC or GA, which one to choose while solving Mixed Integer Programming problem?

I am interested to solve a mathematical problem (MILP) using evolutionary algorithms but confuse about which one to choose as a beginner in the programming languages. Suggest an algorithm easy to...

13 July 2020 4,027 51 View

What is the best tool for adding references (in a research paper) within a word file?

What is the best tool for adding references (in a research paper) within a word file

16 June 2020 8,275 9 View

How can I develop an ANFIS model for WQI in matlab?

I want to implement the Water quality index in ANFIS. I have used constant output, but not getting the desired output.

06 February 2020 3,394 4 View

Can food supply chains be secured by blockchain diffusion???

Blockchain technology promises to provide complete information security and elimination of intermediary alteration. But can we really full utilize this feature to food supply chain. How can we...

03 May 2019 5,476 2 View

When the unit of analysis is 'supply chain' or 'organization', is it acceptable to take more than one respondent from an organization?

Question related to research methodology

06 March 2019 9,150 7 View

No title

02 May 2018 368 2 View

How to generate UDF in Fluent to measure Droplet concentration at a particular downstream in Turbulent flow?

I don't know how to write UDF. If you know then Please help me..or you can also write the UDE for me and Send me.

22 February 2018 1,883 3 View

Can residual centered interactions be used in general linear models?

I have created an interaction that is residual centered. Can I use this interaction in an ANOVA, or should these only be used in regression? Many thanks.

15 January 2018 7,343 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

What is the error of the Txy diagram?

Hi, I know the attached Txy diagram has an error but can't figure it out. Could you please look at the diagrams and explain it to me?

23 February 2021 4,239 1 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

What Model for Vaporization and Condensation in Fluent will be best?

Hello, I am running a simulation that includes water phase changes. These phase changes will take place below the boiling point of water so I need a method that can accurately model this. From my...

15 February 2021 4,973 3 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View

Desalting using DSC- 18 SPE columns?

I am looking forward to remove excess of salt form my metal based porphyrin and trying DSC-18 columns for the same, can someone suggest me how how can one decide the polarity of the solvent to...

01 February 2021 9,076 1 View

What are the thermodynamic and kinetic similarities and differences among dendrite formation during solidification of metals, ceramics and polymers?

Basic dendrite formation of metallic alloys are explained by thermal and constitutional degree of supercooling and most-supercooled band of liquid somewhat ahead of solidification front that...

30 January 2021 8,450 2 View