After we find a gene fusion by performing PCR and later confirm it with sequencing, how do we further confirm the clinical role of that gene fusion in cancer? What should be the methods to follow after find gene fusion/mutation in cancer cell lines?
Thank you for the detailed reply.
Actually I am working on cDNA. Is is possible to use transfzction, proliferation and migration assay for the confirmation?
Sirs ,what about using vector virus with remedy molecule targetting that particular part cell with key for the particular part cell membrane protein.
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