I did the experiment with 2M NaOH in 60°C for 24h, but no hydrolysis occured. Can anyone advise me on how to improve the protocol to achieve the successful hydrolysis of polyacrylamide?
Hydrolysis of polyacrylamide you can reflux amide with alcoholic 2M NaOH for 2-3 hrs & then neutralize by HCl to get acid.
Im trying to transfect A549 cells using lipfectamine 3000. im using a 96 well plate with 1microgram plasmid DNA in each well . should i remove the culture media or change it or decrease it befor...
21 July 2024 7,610 2 View
Suppose, there is a square region of length L in the 2-d plane. N points are chosen from inside the region uniformly randomly. Consider these N points as the vertices (nodes) of a graph (network)....
26 July 2021 649 3 View
Has anyone tried to do langendorff free adult mice cardiomyocyte isolation following this protocol? https://www.ncbi.nlm.nih.gov/pubmed/27502479 I have few questions to ask about plating the...
05 February 2018 4,450 2 View
Additionally, I would like to receive literature about fine morphology of Caloneis group [email protected]
21 September 2016 5,205 4 View
There is a global trend towards an aging population. In this context it is questionable whether the age limit in employment could be a discriminatory criteria.
02 March 2015 5,940 4 View
We are studying a material of Hyalosira delicatula but we cannot find studies about its morphology except those mentioned. We would be very thankful if someone could help us. Thank you in advance.
16 August 2014 7,373 5 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,936 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,105 4 View