How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

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26 December 2020 1,951 3 View

How to determine the total and specific IgY?

Comparing the results of two ELISAs We are working with a solution of purified egg antibodies (IgY), both specific (polyclonal) for certain microorganism and non-specific. The problem is, we want...

12 August 2020 3,327 2 View

Why is my bacteria not growing after a transformation?

Hello, I did a transformation a few weeks ago and while only a few bacteria grew, I was able to pick single colonies for a liquid culture. However, I had to repeat this experiment because...

28 July 2020 278 6 View

Any suggestion of bibliography in a wide sense (papers, guidelines, systematized experiences) about "male" parenting (fathering)?

Any suggestion of bibliography in a wide sense (papers, guidelines, systematized experiences) about "male" parenting (fathering) and in special in the context of children and adolescents with...

23 February 2020 8,306 3 View

How to derive reflectances from a Calibrate Panel?

I am working with a hyperspectral camera (Headwell) and I get the DNs from it. I don't have any calibration files of the camera. Do you consider that is possible to estimate reflectances using a...

18 November 2019 2,915 1 View

Why is my tissue detaching after H&E staining?

Hello! I've been having trouble with staining lung and pancreatic tissue. While doing H&E, tissue partially (or completely) detaches. Sometimes, the tissue folds on itself as well. Not sure why...

31 October 2019 8,432 4 View

From whole mitochondrial DNA sequencing data of paired peripheral blood /tumor samples, which is the best algorithm to identify somatic mutations?

We are using SRELKA to identify somatic mutations from whole mitochondrial DNA sequencing data of paired peripheral blood /tumor samples. However, we have noticed that the somatic mutation list...

12 August 2018 1,406 2 View

What is the best probe height to measure Caspase-3 with a fluorescence kit using Synergy H1 microplate reader?

Hi, I'm measuring cellular Ca-3 activity with a fluorescence kit (EnzChek Caspase-3 Assay Kit 1) using a Synergy H1 microplate reader. With this current fluorescent kit the final volume to...

01 May 2018 8,793 4 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How can I separate Gertrud Pfister's profile and the profile of Gerald Gems?

we are co-authors of several publications. But I am the only and single author of most of my publications which are based on my own research. How can I separate the profiles?

21 February 2021 4,582 3 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

Change of First name?

In some publications you refer Deivid Chica (with Barranco) but the true first name is Maria Dolores Chica , in spanish Ma. Dolores Chica. Before I answer yes is an author Deivid Chica, but...

18 February 2021 5,004 3 View

How should I compute a new variable with z-scores?

I would like to create a new variable using 4 different measures: hIndex (range: 1-47), # of publications (range: 0-111), and 2 other measures (range: 1-7). I changed all of these variables into...

09 February 2021 4,866 3 View

Do you know any reporting tool, guidelines for credibility assessment tool used for studies/publications with Artificial Intelligence?

I want to conduct a Systematic Review and assess the quality of methods used/proposed by researchers. I found out that scientists often utilize QUADAS for that...

05 February 2021 8,743 5 View

How do Research Gate proceed to integrate in the reference data publications and citations the recently published journals ?

How do Research Gate proceed to integrate in the reference data for publications and citations the recently published journals - for instance for the last months of 2020 and for instance Intl J...

02 February 2021 9,336 7 View

How to add journals not listed on Research Gate ?

I have two publications one listed in the web of science master list " European Online Journal of Natural and Social Sciences " ISSN: 1805-3602 and Second " Journal of Managerial Sciences"...

26 January 2021 7,158 7 View

Can you recommend a good book on analytical chemistry for non-chemists?

Dear colleagues, I am asking for you recommendation: I need a book on analytical chemistry in environmental sciences. As a non-chemist (a biologist) I have some basic chemistry knowledge but not...

25 January 2021 8,486 8 View