How can I numerically solve the dispersion equation below using matlab?

The dispersion equation: (cos(w*h1/Ct1)*cos(w*h2/Ct2) - 1/2*((rho1*Ct1)/(rho2*Ct2)+(rho2*Ct2)/(rho1*Ct1))*sin(w*h1/Ct1)* sin(w*h2/Ct2) = cos(k*h)) where omega, w is a function of k. And also k...

10 May 2020 3,262 3 View

What is the future of Nanotechnology in the Wine industry?

Nanotechnology is the branch of technology that deals with dimensions and tolerances of less than 100 nanometres, especially the manipulation of individual atoms and molecules. However, there are...

30 October 2018 4,808 3 View

Economic Effect of Fuel Subsidy Removal and Cost Reflective Tariff in Nigeria

The Centre for Econometric and Allied Research (CEAR), University of Ibadan wishes to conduct a survey-based study aimed at assessing the economic effect of the recent removal of fuel subsidy and...

01 January 1970 766 2 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

Luciferase assay without luminometer?

Hi everyone. I intend to carry out a luciferase assay for my research but have no access to a luminometer. Is there an alternative assay that I can use for my research or can anyone suggest a way...

05 December 2020 9,749 2 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

Luciferase assay for gene-miRNA interaction: which vectors should be used?

23 November 2020 2,813 2 View

Does anyone have experience in TOPFLASH assay in HEK293T cells?

I did lipofectamine transfection in 12-well plates with 0.5M of cells and 0.8ug of TOPFLASH plasmid in each well. After 24h, I changed to media to serum-free media supplemented with 10uM...

20 October 2020 3,584 2 View

Luciferase assay for miRNA-gene: How to construct the mutated prime?

I have experimental evidence (qRT-PCR and WB) to suggest a miRNA downregulates a gene. To test if the regulation is direct I want to perform a luciferase reporter assay. So I designed a primer...

15 October 2020 7,641 4 View

What is the time required for an enzyme or an protein molecule to consume an ATP molecule during a reaction?

In what time do a protein body consumes an ATP molecule for an active process to happen?

02 October 2020 8,135 4 View

Will Cypridina Luciferase (CLuc) Catalyze Beetle Luciferin?

Hello, I am using a lentivirus encoding CLuc to transduce target cells. I want to then lyse the cells in the presence of luciferin and measure luminescence. The kit which I had hoped to use...

15 September 2020 6,664 2 View

How to perform cell transfection and Dual Glo assay with low-adhesive (HEK293) cells?

Hello everyone. I need to use Promega Dual Glo assay for HEK293 cells, but I am really afraid of wash out my cells from the 96-well plate, and at the same time, I am afraid to use coating because...

09 September 2020 4,817 7 View