How can I calculate the relative expression in a transgenic plant?
I transferred a gene to Arabidopsis through the floral dip method (this gene is not present in Arabidopsis). I studied the expression of this gene in real-time. Can I use one of the LIVAK equations for it?
My data includes the reference gene and the target gene. I know that one of the lines with the lowest expression and the highest CT can be considered as a calibrator, but it doesn't make any sense to me, because treated and non-treated are in fact the same, what is your suggestion?
The relative expression level can be calculated by the 2−∆∆CT formula as previously described (Livak and
Schmittgen 2001), with a gene as internal reference standard
Dear Zahid Hussain
Thank you for your answer.
2-𐊣𐊣CT [ (CT gene of interest - CT internal control) sample - (CT untreated sample - CT internal control)]
My data just covers the first part . My data only includes the first part of the equation. A gene as an internal control (actin) and the gene of interest. I have no data for the second part of the equation. If I consider one of the lines as a control, can I count it as an untreated sample?
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