How do I estimate the phase distribution of a dilute solute using vapor pressure?

What is the most fundamentally accurate way to calculate the distribution of a solid between the condensed, vapor, and/or liquid phases? Let's say I have a very small amount of elemental silver,...

22 December 2020 8,060 0 View

How much time is required to use TissueLyser LT ?

I am using TissueLyser LT to extract DNA from plant tissue ( without using liquid nitrogen cause it's not available ) but I do not know how much time is required? I did this in 6 minutes at 50HZ...

15 August 2020 9,270 7 View

How we can separate uncut plasmid form cut plasmids without running on agarose gel?

how we can separate uncut plasmid form cut plasmids without running on agarose gel? for improving the concentration of purified long plasmid

02 September 2019 4,191 3 View

How to simulate different types of IoT networks and connect them together?

Hello, guys. I have to simulate different types of IoT networks and connect them together. At the first stage, I have to simulate these networks: 1) A group of nodes which are by Zigbee. 2) A...

22 June 2019 2,362 14 View

What antibiotics should I use?

23 January 2019 1,641 3 View

How can we arrest cancer with a new model of wifi waves? Those waves must have what special trait?

I mean, new wave for peace, should have a what new trait? For example, religious mans say the meditation can help peace (with positive waves) and sometimes they are right!! Can you help me for...

22 October 2018 9,220 9 View

Does anyone know about immunological changes happening after inducing pancreatic beta cell ablation in zebrafish? is there any literature for it?

zebrafish immunological response after inducing cell specific ablation in zebrafish

06 March 2018 7,967 1 View

What are the reasons of primer dimer formation in Real Time PCR?

I am working on gene expression. In Real time PCR experiment, a primer dimer is observed.

30 September 2014 8,180 9 View

How can I model epoxy-concrete interaction in near surface method FRP in Abaqus?

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24 February 2013 252 1 View

Could anyone let me know how to express a 32 amino acid peptide in E.Coli please?

I want to produce and refold 32 mammalian amino acids that as a small protein in E.Coli. Has anyone got experience and suggestion which pET vector is suitable?

15 May 2012 9,664 8 View

Most appropriate fit of a standard curve in an enzym-linked lectin assay?

Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...

03 January 2021 1,010 6 View

How to solve carry-over and interference problem when detecting tulathromycin by LCMS?

I am working on the determination of tulathromycin in rat faeces using QE-focus (Thermo Fisher)(Waters BEH C18, 2.1x100mm, 1.7um)(0.1%FA+H2O, 0.1%FA+ACN) . I encountered two tricky problems....

02 January 2021 2,713 5 View

No title

It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

Hi, i'm trying to synthesis Graphene oxide without (NaNO3), how ever at the end of the synthesis it just black dusty solution, any idea?

here is step from journal. 1. add 3g graphite powder in 70ml H2SO4. in ice bath and constant stirring. 2. add 9g KMnO4, after a while it become green 3. heat it to 40celcius for 30minutes, it...

22 November 2020 9,622 3 View

Why the protein is not secreted to the medium?

Dear all, Why is a protein, which is expressed in HEK293 cells, not excreted to the medium? The protein remains soluble into the cell. Which could be the reasons? Thanks for your time! Best...

04 November 2020 4,398 8 View

Antibiotics removal efficiency in WWTP and Fexcreta?

I am calculating the PEC for selected antibiotic compounds and am having problems finding the readily-available online data about the Fexcreta value for my target compounds (Since the PEC...

02 November 2020 7,675 6 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

What is Dopamine?How it affects human behaviour &directly connected to neuro-psychological disorders?

We all known Dopamine is a happy/joyous hormone secrets from brain.How it changes the over all human behaviour & responsible for the well being of humanbeing. Regards, Md Osim

02 October 2020 582 4 View

What solvents can be used to dilute the Lepidopteran defence droplets (scoli glandular secretions from lepidopteran larvae)?

I am trying to analyse the chemical profile of defensive droplets from the lepidopteran larvae (T Trypanophora semihyalina ). when i collected and tried to dilute in the hexane and acetone the...

23 September 2020 2,994 3 View

What's the secret behind deep neural networks efficiency ?

21 September 2020 1,763 4 View