Please help me with the separation protocol of stool supernatant. This supernatant should contain all the secretions and bacterial elements of the feces.
You can try with high-speed centrifugation technique
I am wondering how to set up Cardiac fibrosis in vitro. I will appreciate all your comments.
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I applied the real-time product on 2.5% agarose gel and only targeted the specific band, but even with changes in primer concentration, cDNA, and temperature gradient, these shoulder are still...
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I need a rapid test for coldest in vegetable oil . Or How to predict the appearance of turbidity in vegetable oil?
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Could Vaidya space-time be regarded as the evaporation of black holes? we know that ingoing Vaidya is considered as the formation of black holes. How about the outgoing Vaidya as the evaporation?
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I have a soil block that includes some resonators. There is a problem with selecting just two sides of my block instead of selecting the whole block in PML selecting box in COMSO(when I wanna...
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07 August 2021 8,839 4 View
What is the most fundamentally accurate way to calculate the distribution of a solid between the condensed, vapor, and/or liquid phases? Let's say I have a very small amount of elemental silver,...
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I want to know in the cold weather for improving performance and immunity which nutrient is added to the diet.
22 November 2020 2,454 4 View
I am using TissueLyser LT to extract DNA from plant tissue ( without using liquid nitrogen cause it's not available ) but I do not know how much time is required? I did this in 6 minutes at 50HZ...
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I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
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I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...
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Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
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Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
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What is the secret behind the success of the researcher named Dariusz Prokopowicz who has a "Research Interest Score" of 11,228 and has 2,620,101 reads in ResearchGate. How was it possible for him...
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Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
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We are planning to collect saliva from rats for protein analysis. The issue is that the amount secreted is very low, which makes it difficult to analyze. To increase salivation, we purchased...
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Any pointers on how to get Cryptosporidium sporozoites to glide on non-cell substrates? We've tried oocysts from a couple of different sources (WaterBourne and the production lab at U of Arizona,...
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What are the medical uses of Atropine?The Role of Atropine in Modern Medicine: Indications, Admini... Atropine has several medical uses across different fields of medicine. Some of the primary...
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