Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Why can`t I isolate RNA from Extracellular vesicles?

Hello, I have been trying to isolate RNA from EVs (from MSCs) for a while. I isolate the EVs using SEC. Then I use the "Total Exosome RNA and Protein Isolation Kit" from Invitrogen. When I...

21 October 2020 8,559 4 View

How to directly use a directory of .h5 files as the training set in Python without appending them to a list?

I am trying to append .h5 files saved in a directory for my training set using Python, but I face memory error as the Data set is very large. The following code is what I used for...

23 April 2020 9,649 2 View

To record trajectory tracking performance of a controller, is it resonable to calculate the mean_square_error or the normalized-measn-square-error?

I some papers, the mean square error is considered. In some other, the mse is normalized by dividing the error by the (total sampling instants x the total length of the reference...

25 February 2020 784 4 View

What is the most reliable guide for identification of alternaria species based on morphological and cultural criteria?

what is the most reliable guide for identification of alternaria species based on morphological and cultural criteria?

02 December 2019 174 1 View

How the nonholonomy of mobile robots allow control of the system with less control inputs?

In "Simultaneous Stabilization and Tracking of Nonholonomic Mobile Robots: A Lyapunov-Based Approach", the authors stated that the nonholonomy of mobile robots allows control of the system with...

27 May 2019 7,721 4 View

How to interpret DMA?

18 May 2019 631 6 View

What are the most reliable tools or softwares for designing of the CRISPR gRNAs?

Dear all, Which tool is more reliable? CHOP CHOP or CRISPRdirect? I need some information about other tools and softwares. Thanks a lot

08 May 2019 10,004 2 View

If you could recommend one significant change to make Higher education more affordable without compromising its worth, what would it be?

Higher Education cost is increasing and students encounter some challenges to afford it.

21 March 2019 7,173 6 View

How to match reflection in both visible and infrared?

I have a thin film of a fluorescent material and want to make a reference sample with similar reflection in both visible and infrared to do the comparison. How can I make the reference sample?

26 February 2019 6,857 3 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View