Read the papers mentioned below... you will also gain insights into PGPR and how plants respond to salinity stress.

https://pubmed.ncbi.nlm.nih.gov/37692403/

Article Multi-trait Halotolerant Plant Growth-promoting Bacteria Mit...

Article Zero budget natural farming components Jeevamrit and Beejamr...

Thankyou Sir, for providing this paper.

Step-by-Step Protocol (Using KI Assay):

1. Sample Preparation:

• Weigh 0.2–0.5 g of fresh plant tissue (e.g., leaves).
• Grind in 5 mL of cold 0.1% (w/v) trichloroacetic acid (TCA).
• Centrifuge at 12,000 rpm for 15 minutes at 4°C.
• Collect the supernatant for H₂O₂ measurement.

2. Reaction Mix:

• Mix: 0.5 mL of supernatant, 0.5 mL of 10 mM potassium phosphate buffer (pH 7.0), 1 mL of 1 M potassium iodide (KI).

3. Incubation:

• Incubate the mixture in the dark for 10–15 minutes at room temperature.

4. Measurement:

• Measure absorbance at 390 nm using a spectrophotometer.

🧪 Calculation:

Use a standard curve prepared with known H₂O₂ concentrations (e.g., 0–10 µmol/mL) to determine the amount in your sample.

Then calculate: