Which is the best solvent for dissolving the silver paste that will be used as a conducting material?

27 July 2014 4,453 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

What are the things you should keep in mind while designing a controller for a quadrotor and want to implement it on hardware (via arducopter) ?

I am designing a flight controller for a quadrotor. At first I am designing a nested/cascaded controller consisting of only proportional controllers Kp . Now, if i tune the rate controller for 10...

03 February 2021 5,001 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Using the Taqman PCR, how does one know which allele is being determined by VIC / FAM?

I am exploring a number of SNPs, unfortunately although the test are ok, I am perplexed because my preliminary results are nearly all against what is reported in the literature. To confirm my...

29 January 2021 3,905 2 View

Can't run my IPMSM drive system with DC-supply higher than 125 V. Have anyone had similar problem?

Hey, I try to run my 4 pole IPMSM with PWSVM and use a typical controller with PI-controller with a current loop and speed loop. When I run my system with DC-supply 100 V with a steady-state speed...

28 January 2021 7,354 8 View

How does artificial intelligence impact cybersecurity?

With emerging technologies, the availability of data at every point of customer interaction has increased. Consequently, the need to monitor and secure the data has increased. With data being...

19 January 2021 7,944 9 View

How important Sustainable and Renewable Energy studies and dynamics in Europe ?

Dear sustainable and renewable energy researchers, I aim to review Research areas regarding Sustainable and Renewable Energy and I am currently establishing all the controls. Can you help me to...

11 January 2021 1,099 3 View

Why does HIF-1alpha in HUVECs treated with CoCl2 show two bands on WB?

Hi, I want to detect HIF-1 alpha in HUVECS treated with CoCl2 at 0, 50, and 100uM. I observed two bands at 100kDa (band #1) and 90kDa (band #2) in the treatments and the controls. However, the...

30 December 2020 9,632 1 View

Problems with allele speration by cloning in pKS plasmid?

I am trying to do the allelic separation of a ~8 kb region using cloning strategy with pKS plasmid. However, when I analyze the result of the colony PCR sequencing It seems that both alleles were...

14 December 2020 5,829 2 View

Did we interpret our SDS-PAGE results correct?

This is our first time doing SDS-PAGE, and therefore we would like to hear from more experienced people if we interpret the results right. Attached is a picture of one of our gels with our...

01 December 2020 1,440 3 View