How can I calculate alpha amylase activity from bascilus sp. without having absorptivity coefficient?

23 September 2021 1 5K Report

I Need the formula to calculate amylase activity from bacillus. Can please someone guide me on how can I do that?

Read the following method

Enzymatic Method for Determining Amylase Activity (Amylase Activity Assay)

https://www.sigmaaldrich.com/IN/en/technical-documents/technical-article/protein-biology/enzyme-activity-assays/amylase-activity-assay-kit-mak009

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