The MRS and YPD both the media are showing antioxidant activity almost the same as probiotic culture supernatant. Is there any method so that I can avoid this issue? Or Can I make a sterile suspension of cells in distilled water and keep it for some hours and use that?
I have done three assays,
ABTS, DPPH and FRAP
You can Resuspend your cells at sterile deionized water or phosphate buffered saline PBS and use them for the control sample instead of MRS broth.
Kindly check https://www.hindawi.com/journals/ecam/2018/1756308/
Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5452251/
Mohamed Kishk First, I would like to thank you to getting involved in my query. Actually, I am studying the antioxidant property of two samples
1- Bacterial culture supernatant that I have collected via centrifugation after 48 hours incubation.
2- Whole bacterial cell suspended in PBS/DW collected after centrifugation.
My problem is that I have taken MRS media only as a negative control to remove the extraneous variable due to media in my supernatant. But my results are showing that negative control (MRS only) is more antioxidant than supernatant.
Chinaza Godswill Awuchi Thank you Sir for the references, I have read them. As I mentioned my query in detail to Mr. Mohamed. Please go through them and suggest the possibilities.
Chinaza Godswill Awuchi Sir, the review paper you have given me is very important to me
You can referArticle Antagonistic trait of Staphylococcus succinus strain AAS2 ag...
for DPPH and Hydroxyl radical scavenging property as they have standerdized it for probiotics- Badges
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