How can I avoid crystals in skeletal muscles frozen section?

10 November 2016 1 319 Report

I used cold isopentane and OCT to froze my tissues (muscle-TA).

It is not clear what is your purpose. If you want to use cryostat to make sections for your samples I don't think there will be crystals in your samples if you use fresh muscle samples. Cool down your isopentane in liquid N2 and then freeze your muscle block (2cm X 1 cm). I used acetone frozen in dry ice to freeze my muscle samples for muscle fiber typing.

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