How can i attach an oligomer into my vector to create a site?

27 February 2016 1 5K Report

Its a binary vector and i need to clone my gene into the vector and i attached an addition site into the oligomer so that my gene can be rescued from digestion.

Falseplanet Jia

Hi,

You can choose to use overlap PCR and/or gibson assemblly to fullfill you goal.

Good luck!

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