Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

When sequencing a plasmid to confirm transformation has been successful, would I have to sequence the full plasmid?

I have transformed some DH5alpha cells with point mutated proteins placed in pcDNA plasmid and extracted the plasmid; now I have to send the plasmid for sequencing for confirming the presence of...

19 January 2021 5,842 3 View

How can decrease the fluorescence background for Raman Spectroscopy?

Could you help me with, how can I decrease the fluorescence background for Raman Spectroscopy?

18 May 2020 7,685 11 View

What are the differences between Raman spectroscopy of amorphous and crystalline carbon materials?

What are the differences between Raman spectroscopy of amorphous and crystalline carbon materials?

30 April 2020 3,435 8 View

How to separate the Raman spectroscopy peak?

In the result of the test for Raman spectroscopy, there are many broad peaks that including a lot of information. In fact, How can I correctly separate those peaks?

26 December 2019 3,460 3 View

What is the best way for poor Nations to conserve thier water resources especially with the current trends of climate change?

I am developing a research on the link between poverty, natural resources management and climate change in Nigeria and need some guidance.

11 December 2016 7,577 0 View

Can i use b-actin in real time PCR?

can i use b-actin as control gene in real time PCR? because in articles that i have, they use GAPDH as a control for real time pcr and b- actin for western blot. i do not know why and what is...

01 May 2016 5,781 10 View

What are the basic fertility destructive factors of tropical soils?

What are the basic fertility destructive factors of tropical soils? Temprature; Effluents; Compaction?

02 March 2016 8,868 17 View

What is the most sustainable way of managing the problems of sawdust and wood shavings accumulation in our cities?

Biological? Physical, or chemical?

07 August 2015 9,700 3 View

Can exploratory factor analysis be performed on a multiple choice question?

10 March 2015 5,728 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View