I put electrode in PBS solution and then i applied CV about 0.5 to 0.9 v in 10 times .... graphite surface can be oxide with this method ? and can i immobilize enzyme in this surface ?
Dear Abbasali,
In my opinion one of the best ways of activating graphite electrodes is by polishing in emery paper and then dropcasting the enzymes directly. There is a vast amounts of papers using this method and describing the methodology.
I am not so sure if you are creating oxide species at the potentials you are using with PBS. Can you see any peaks in the potential window you are using? In that case you might be creating some oxides.
We can activate the graphite electrode by anodic oxidation in phosphate buffer medium. One of my friend Dr.G.Ilangovan did surface activation process for GCE. The same strategy you can adopt for graphite electrode too.
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