25 July 2023 0 8K Report

Hello. Can anyone help me with a link to download or buy UVWin 5 (or 6) Software for UV-Visible Spectrophotometers.

Thank you!

More Rózsa Sándor's questions See All
How to realize asymmetric bilinear mapping between GF(2) as base field and GF(2^m) in PBC library ?

Hi to all, I wish to implement an asymmetric bilinear mapping using a non supersingular curve defined over binary finite field. The two subgroups over the same curve are GF(2) that stands as base...

23 November 2023 8,262 0 View

How can I access European Carabidae trait data?

Unfortunately carabids.org is down. I am looking for European Carabidae traid data, like Distribution range size, adaptability to environment, habitat preference, body size, hind wing morphology,...

30 March 2022 825 3 View

C710 cells and Eilat virus. Plaque assays are not forming. Cells look washed away. Overlay media is the problem?

I'm trying to produce a plaque assay with an Eilat virus and C710 cells but whenever I go to stain it seems like the cells are just washed off the wells and I get nothing. I am using the correct...

20 December 2020 3,962 0 View

Tips for Murine Small Intestinal Organoid Monolayer Culture?

I am isolating crypts from the jejunum of black-6 mice and growing them into 'spherical' apical-in intestinal organoids. I have had lots of success growing these 'spherical' organoids in a 40 uL...

17 September 2020 2,808 1 View

Membrane protein missing from SDS-PAGE gel?

I am trying to run an SDS-PAGE after his-tag purification of a sample. The protein of interest is an oligomerising membrane protein with an YFP tag attached. When the fractions of the sample from...

07 September 2020 3,461 6 View

Qiagen Viral RNA extraction kit showed similar results for positive and negative control. How should I proceed?

Our team is looking for viral RNA in the environment. We took swabs off of doorknobs using cotton dipped in water and then submerging that swab in viral transport media. We also wiped 1 surface...

08 June 2020 6,731 3 View

What is the acceptable amount (%) of plant amplicon during ITS metabarcoding of endophytic fungi from a plant host?

During metabarcoding of endophytic fungi, many seem to use ITS primer pairs that are not (fully) selective for fungi. We experienced that such primers also amplify the ITS region of an in vitro...

09 March 2020 3,429 2 View

Sterilization of glass pipettes?

Hi all i am using glass serological pipettes and would like to sterilize them in an autoclave. How should i prepare them beforehand, so they get sterilized but i can take them out and move without...

09 December 2019 3,729 0 View

How large are average tobacco leaf protoplasts?

I am trying to sieve tobacco protoplasts and looking for the appropriate mesh/pore size for cell strainers. I have seen papers recommending a range of sizes from 50-150um - but it would be easier...

16 May 2019 3,873 0 View

Could someone help by reviewing a manuscript?

Hi everyone! This paper keeps getting rejected, and I could really use some help. Is there anyone who could help me out with a quick peer review? The manuscript is available as a preprint here:...

03 June 2018 443 4 View

Similar questions and discussions
Is there a method to measure the concentration of Cs, Sr, Co using spectrophotometer?

it's important to know that we are using the inactive elements of Cs, Sr, Co

25 July 2024 1,553 2 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Hi. Can the absorption measurements of a polymer in a solvent be considered reliable when using a spectrophotometer?

zzz

02 July 2024 918 2 View

I want determine Iron by spectrophotometer? Anyone know about the spectrophotometric determination of iron and copper, kindly share the procedure?

spectrophotometric determination of iron and copper

04 June 2024 1,720 0 View

NanoDrop 3300 Error 8008?

Hi everyone, has anyone else experienced an 'Error 8008' with their NanoDrop 3300 (image attached), on a laptop which it previously worked fine on? I have tried uninstalling the software and...

02 June 2024 9,025 3 View

How shall i consider extinction coefficient unit?

I want to determine the conjugated drug to IgG ratio by nanodrop. I know the extinction coefficient of IgG and drug by M-1cm-1 but all the unit in the nandrop program is based on g/ml and so on...

15 May 2024 9,097 4 View

How to increase the 260/280 and 260/230 ratios in DNA extraction?

I manually extract DNA using a modified Sambrook protocol (phenol-chloroform), from tissue samples (skin or muscle) of stranded animals even at a high state of decomposition. When I take Nanodrop...

05 May 2024 5,588 1 View

Protein Inhibition Denaturation Assay: No absorbance detected at 660nm?

Methods are based on Chel-Guerrero and colleagues'Phytochemical Profile, Toxicity, and Pharmacological Potenti... . We use a 1M Tris buffer with pH of 6.8, with Bovine Serum Albumin as the...

25 April 2024 8,802 0 View

PAGE with urea for miRNA integrity?

Hello, I'm trying to elaborate a denaturing 12% polyacrylamide gel (19:1) with urea 8M in order to visualize the presence of plasma total miRNAs, I'm using TBE buffer, my extraction (Trizol)...

22 April 2024 1,740 3 View

Why does the doxorubicin in pbs solutions are giving variable spectrophotometric readings at similar concentrations and similar pH?

Variable readings at same time point and similar solutions

21 April 2024 743 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close