What is the recommended/optimal time for resting mouse splenocytes after thawing?

This is my first time working with mouse cells. We isolated and froze down in 90% FCS and DMSO. I would like to retrieve them and do immunophenotyping and intracellular cytokine staining

03 January 2021 6,399 1 View

Can CMOS and CCD images sensors be used as microphones?

I was thinking about Msps and Gsps image sensors in different environments. If a pair of identical image sensors - one exposed to air, gases, plasmas, or partial vacuum - and one in a good...

21 December 2020 9,747 2 View

Is there a tool to find the closest species with an assembled genome for any given species?

As more and more genomes are being sequenced the likelihood of having a close relative being sequenced is rising. So lets say I have a non-model species Chela cachius and I want to find the...

25 November 2020 6,267 4 View

Where can I find a longterm global mean EVI/NDVI dataset?

I am looking for a longterm (10+ years) dataset of mean EVI/NDVI or annual maximum EVI/NDVI and similar. Basically, I am trying to avoid downloading tons of images and doing the averaging myself,...

28 September 2020 8,374 2 View

What would be the criteria for an expression vector designed for S. cerevisiae to also work in S. pombe?

I am interested in using a S. cerevisiae expression vector such as the pYES2-DEST52 plasmid for transgene expression in S. pombe. This plasmid has the GAL1 promotor, so I would like to make sure...

18 September 2020 7,460 2 View

Can we infuse drug along with sedative?

I would like to infuse rats with drugs of our interest. However, we also need to keep animal under sedation by administrating pentobarbitol via jugular vein with the help of syringe pump since we...

15 September 2020 7,501 5 View

What is a suitable solvent for extraction of arsenic?

Hi. I'm doing some work on arsenic speciation. I am following a paper titled, "SPECIATION OF ARSENIC IN A CONTAMINATED SOIL BY SOLVENT EXTRACTION." which calls for the use of chloroform or benzene...

06 September 2020 6,351 4 View

Ideal saline volume for intravenous injection in rats ?

Hello everyone, my interest is to understand the molecular mechanisms involved in anaphylactic shock using Wistar rats. While i inject non-allergic naive rats with iv bolus (1 mg ova in 1 ml),...

27 August 2020 9,444 2 View

Does mysticism have a scientific basis?

Mysticism is often treated as the opposite of science. But is it? Please see Natural Laws and Mysticism in the Cosmic Cycle of Creation

25 July 2020 6,696 80 View

Which molecular dynamic simulation software is more applicable for protein and large-area surface interaction simulation? And how to run it?

Dear experts: I am trying to do the molecular dynamic simulation for protein-surface interaction, the surface (like cellulose or PET) is a film-like or crystalline material with large surface...

20 March 2020 3,960 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View