Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

How can I improve RNA extration from human neutrophils?

I tried several times extrating RNA from human neutrophils and the amount of RNA at the end of the precedure was minimal. I also tried using colunns from QIAGEN. Never had any problem with tissue...

26 January 2021 8,881 4 View

A light for the oxford nanopore rapid attachment chemistry. Does anyone know how this works?

Some Oxford Nanopore kits have Primers with 5 ' modification that provide ligation to adapters without the need for ligases (ligase-free method), as in 16S barcoding kits. Does anyone know what...

21 December 2020 8,612 3 View

How can I merge Scopus and WOS metadata?

I am conducting a systematic review and need to merge the metadata of the articles resulting from the search in these databases (Web of Science and Scopus) to analyze in WOSviewer. How can I do this?

17 December 2020 9,292 3 View

When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

Can we use efficiently capillary zone electrophoresis (CZE or FSCE) to separate nanoparticles?

Can we use capillary zone electrophoresis (CZE or FSCE) to separate nanoparticles, please? Are there works in the literature on this topic? What are the main difficulties to develop...

04 September 2020 3,385 5 View

How easily do you identify a predatory journal?

Predatory journals are spreading and producing more and more new ways to attract good authors to publish your articles. So, how can we identify them? How to avoid them? And how to create systems...

09 August 2020 4,146 13 View

Can anyone help to model CFST step by step in abaqus ?

i am trying to model concrete filled steel tube column on abaqus. but i don't find the accurate result and i dont know where the problem is coming from

24 March 2020 1,892 3 View

How can I assess a method that returns high-expressed genes?

Hello, I working with a method that finds high-expressed genes in a single-cell experiment. How can I assess such method? I know I can compare it with the ones in literature (t-test, regression),...

22 February 2020 3,023 2 View

What is the best analytical (accurary and precision) method for reduced glutathione from yeast extract?

I would like to know what is the best analytical method for measuring glutathione? I already saw some methods like colorimetric (spectrophotometer), HPLC, LC-MS, electrophoresis. Also, what is the...

28 January 2020 3,461 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View