During transformation of Agrobacteriumu with plasmids ,PCR Positive colonies are not POSITIVE after restreaking.. Why?

Initially we are getting positive amplification .The positive colonies are selected and restreaked.The PCR is not showing positive amplification.

18 December 2020 9,802 3 View

Raman cross section?

Can anyone help me to calculate Raman cross section in RE doped glasses?

12 December 2018 6,330 1 View

Which is the best kit/enzymatic method Efficient for the isolation of cells from the mouse or human tumors?

I want to isolate the cells from the tumors (mouse model and human). I read that Collagenase I is harsher than Collagenase IV. Can anyone please suggest if there is any commercial kit with the...

08 August 2018 4,961 2 View

Hi Everyone, can anyone tell how to get growth kinetics of bacteria with the help of Hoechst stain??

Hoechst 33258 stain

15 May 2018 3,128 3 View

Can any one give Jcpds fileof ZnSe and CdZnSe?

i have obtained an additional peak in XRD at 31.7 degree. so i want to get the idea about this peak few articles mentioned it but not showing angle values.

29 November 2017 3,772 3 View

RAW cell culture and FACS?

Hi folks, I am growing RAW 264.7 cells in 12 well plates, RPMI with FBS and PS. When harvested and acquired cells in FACS, in few of the stimulated wells population is missing or second population...

23 August 2016 9,977 3 View

How I get draw geometry of atomic crystals for atomic position for siesta input?

I am interested in nano clusters of CdS, but unable to draw structure. how I get atomic positions?

09 June 2016 7,476 2 View

How to increase return loss in mircrostrip patch?

patch design for 2.4 with coxial feed

08 March 2016 2,996 8 View

Can anyone help with the observed hysteresis in in-situ conductance measurements?

During in-situ conductance measurements, the gate potential was varied from -0.8 V to 0.6 V (anodic cycle) and reverse measurements from 0.6 V to -0.8 V (cathodic cycle). I have observed a huge...

21 December 2014 2,314 3 View

Can somebody help with In-situ conductance measurements of chemically synthesized conducting polymers such as PEDOT-PSS?

I am using gold interdigitated electrodes with 5 um width and spacing. I spin coat PEDOT-PSS on the IDE to get around 100 nm thick films. In-situ conductance measurements are done by dipping the...

18 November 2014 9,169 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View