I am searching for a well defined protocol for Arabidopsis growth in a growth chamber with respect to following things:
1) Optimum temperature, light, light-dark cycle, humidity
2) How plants could be grown pots with potting mix? (Any requirement of covering of pots, trays? Watering methods/requirements etc.?)
etc.
Anyone to help with this?
Hi,
Here you can find a well described protocol for soil and growth medium recipes. Also protocols for seed sterilization. https://bio-protocol.org/bio101/e126
Regarding the growth conditions depends on your interest but 60–100 μmol m–2s–1 of cool white fluorescent light, temperature 22°C and 50-60% humidity is our standard. For the photoperiod you could use 8 - 16h (light-dark), 12-12h or 16-8h, short-day, neutral-day or long-day, respectively. It's up to you and whatever you look for as the phenotype of your plants will vary a lot depending on the photoperiod.
Cheers!
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