Suhas Karle

4 Questions 4 Answers 0 Followers

Questions related from Suhas Karle

GUS Quantitative assay calculations?

Can anyone explain the calculation of GUS activity using fluorometric MUG assay. I could not find a detailed explanation of the calculation part. Thanks in advance.

30 November 2022 4,413 0 View

How Arabidopsis plants could be grown in a growth chamber?

I am searching for a well defined protocol for Arabidopsis growth in a growth chamber with respect to following things: 1) Optimum temperature, light, light-dark cycle, humidity 2) How plants...

26 June 2021 5,393 1 View

Why the T0 plants selected with kanamycin are not surviving in the soil?

The Arabidopsis wild type plants transformed with the transporter gene construct (gene + PBI121) has given me the T0 seeds. The T0 seedlings selected with kanamycin (50 mg/L) when placed in soil,...

12 June 2021 9,465 10 View

How we can prevent fungal infection in the Arabidopsis plants after they are transformed?

I have done a floral dip transformation of Arabidopsis plants using the 5% sucrose suspension of Agrobacterium. 5 days after transformation, I observed some fungal growth near the base area of...

09 August 2019 387 5 View