How AGO-2 decides which strand to cleave in siRNA mediated silencing, When the other strand( antisense/guide) has to complement and cleave the mRNA ?

20 July 2020 1 7K Report

AGO-2 cleaves the sense/passenger strand in siRNA and remain bound to antisense/guide strand which compliments and mediates the mRNA silencing.

My question is how AGO-2 decides which strand to cleave.

Athanasios Dalakouras

The strand that exhibits lower thermodynamic stability at its 5′ end will be incorporated into AGO, see DOI: 10.1016/s0092-8674(03)00801-8.

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