When you want to pre-test, you must ensure few things like the pre-test group must be similar to your study group, you must follow the details of your study protocol while administering the questionnaire, and note down the issues during questionnaire fill up. But the main thing is that it should be conducted at a place different to your study place, and more over you should not include its results with that of the final assessment you do for your study. Results of the pre-test will lead to changes, if required, in your tool.
Your study is related to prevalence and finding the risk factor for a particular disease. For your present study, the prevalence in percentage is available or not. First you have to find out. If not available, then you go for the pilot study. I think, this pilot study was only you was mentioned as pre-test. Is it correct? The correct term is pilot study.
The questionnaire you have used the same main study questionnaire then only you will get the correct prevalence and the corresponding risk factors. For pilot study, you have to collect a sample of 50 is enough. But, do it correctly. Then you will analyze the 50 data related to your study. Then you have to get the prevalence percentage and you have to find the risk factors.
I have little different opinion compared to Senthilvel... Pre-testing and piloting are not the same as also pointed out by Muhammad... Piloting is a miniature form of your study... but pre-testing is concerned about testing the tool only... may not be related to sample methodology