Hi there,

Instead using premix master mix Taq polymerase, you should obtain Taq polymerase.

Often supplier provide PCR buffer besides Taq polymerase enzyme. Also you need to buy dNTP mix. Now you can make your master mix and add every additive you want.

Thank you Ghaedi.

It is a part of my coursework for which I am taking exams. So I need to know the concentrations and quantities of additives. If you can provide.

Good starting values for a master mix are given in the QIAGEN HotStarTaq-Plus Polymerase manual, page 15 and following.

Before preparing the master mix, you need to know your number of samples plus 1 positive amplification control plus 1 negative amplification control. Add these up as multitudes of 25 or 50 µl and add 1 additional PCR volume as setup reserve (that way you will be able to take each reaction setup in it's predetermined volume).Mix and spin down the master mix, then distribute it into the cups or wells.

Please see the file in the attachment for the detailed numbers.

Thank you Kulmann.

Its helping for me.