Muhammad Shoaib Akhtar

7 Questions 29 Answers 0 Followers

Questions related from Muhammad Shoaib Akhtar

From where to get .vcf file of known variation of human genome?

For NGS data analysis, I need to get .vcf file of known variations of human genome. I find vcf file at NCBI but couldn't unzip it. How may I get solution to this situation?

07 July 2019 7,619 1 View

What is the difference betweem FM 100 Hue Test and D-15 Test?

Both of these tests are used to screen for colour vision deficiency or colour-blindness.Both these tests also lie under umbrella of Farnsworth-Munsel Test.So I want to clear that where do...

06 June 2015 3,630 2 View

How to make a master mix for PCR?

Master mix is made before running a PCR so NA can be added to it. I just need quantity and procedure of making master mix for PCR.

04 April 2015 9,190 4 View

What can be the maximum difference in melting temperature of primer pair?

I recently read that there should be

04 April 2015 411 19 View

What are the conditions to be optimized before running a PCR?

What are the basic conditions to be optimized before running a conventional and/or real time PCR in a laboratory.

04 April 2015 3,693 8 View

What are the basic steps of Karyotyping?

Karyotyping is a part of my coursework. I neither have performed it nor studied. I want to know about its principle, steps and interpretation. So I can learn about it. Send me some resource or...

01 January 2015 10,011 0 View

How a Questionnaire (Research Proforma) is to pre-test?

A questionnaire for Genetic Epidemiology, Risk Factors and Prevalence Study is to test. Sample Size is 260. What would be the method to pre-test.

12 December 2014 3,324 5 View