Does anyone have suggestion for the house keeping gene to be used in qRT-PCR of bacteria. More precisely, S aureus?
16S gene is often used for E.coli as house keeping gene. But it could be good for S. aureus too
Guy's there are several housekeeping genes can be used in S. aureus.. based on my experienced.. nuc gene is the most preferable in this case.
Regards
Dear Vlado: What I learned about qRT-PCR is that you should test all year genes your are interested in. Afterwards you use 2 or 3 of them which have the lowest difference in your gene expression profiles in your setup. Then use them as "house keeping genes". This is a save way in doing qRT-PCR!!! Cheers, Nadine
Thank you for your help. I had problems with 16s and that was the reason I asked for help. I was looking at gyrase B (might be good choice, at least papers say so), but i guess i will have to test several.
Again, thanks for your input.
Cheers, Vlado
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