I want to use NHP-1 and HNP-2 with my S aureus.
Does anyone have references with good protocols?
I would like to measure HRV in mice. Is it doable with following setup: ECG with sampling rate of 1000Hz and in lightly anesthetized mouse 2% isoflurane? If yes, what are your experiences with...
04 May 2015 2,264 10 View
I found very limited number and any help would be highly appreciated. Thank you in advance.
01 February 2014 8,160 4 View
I am doing experiments with S. aureus and I am particularly interested to quantify catalase activity before and after the treatment. Does anyone have suggestion which assay should I use and their...
08 September 2012 1,604 9 View
I am trying to isolate one S. aureus protein for the follow up production of anti-bodies against that protein. Does anyone have suggestion for good expression vector and protein purification...
07 August 2012 6,446 8 View
07 August 2012 7,272 9 View
Does anyone have suggestion for the house keeping gene to be used in qRT-PCR of bacteria. More precisely, S aureus?
02 March 2012 5,758 5 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...
10 February 2021 5,927 1 View
I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.
03 February 2021 6,959 4 View
Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...
03 February 2021 4,630 10 View
Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...
01 February 2021 7,153 4 View
How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...
31 January 2021 4,528 10 View