Control in microstructure with growth inhibitor?

Dear researchers, I wish to understand the role of grain growth inhibitor in controlling the microstructure of the ceramic pellet. How it controls the growth? Also, I wish to know the grain...

15 January 2021 3,859 3 View

What are the invivo screening methods for anti TB drug?

what are the invivo screening methods for anti TB drug?

25 December 2020 722 1 View

Optimization technique in ball mill?

Hi all, I wish to know about a common problem faced by most of the researchers who are dealing with ceramic materials that my ceramic powder is sticking with the balls after the operation. can...

02 December 2020 8,232 3 View

Which one is best for extraction method MICROWAVE OR Soxhlet for leaf components?

I want to isolate components from leaf material, so I have beter solvets but suggest me method which will improve yield and purity

08 September 2020 1,003 2 View

What is the best method and solvent for isolation of alkaloids from leaves?

my target to isolate alkaloids from plant leaves, as phytochemistry of plant reported for some alkaloid derivatives , so please guide me.

01 August 2020 7,376 15 View

Are there any photonic resonating structures which show a blueshift in their spectral response upon increasing the refractive index of the top region?

This question is more in regard to the resonant structures used for sensing applications, namely surface plasmon resonance, photonic crystals and guided-mode resonance based devices, etc which all...

17 March 2020 6,800 3 View

How to run my model by using double precision executable in Abaqus?

I created a rolling model by using Abaqus/Explicit. All the warnings and errors, I corrected but still have a problem with one warning. The warning is about running the simulation in double...

25 March 2019 6,671 3 View

Does keeping tissue sample (patient biopsies) in Trizol for a long time (>6 month) degrades DNA ? or Leads to sheared DNA?

I am trying to isolate RNA and DNA from the tissue biopsy stored in Trizol at -80 degree for more than 6 months . I isolated RNA using the standard Trizol method and obtained good quality and...

22 November 2018 2,446 2 View

What is the difference between 2(-ddct) and fold change and relative expression?

I have calculated 2 (-ddct) for a set of 6 genes in 5 tissues using GAPDH as reference and one of the tissues as the calibrator. However, after computing the 2 (-ddct) values, which I believe is...

21 August 2018 3,108 3 View

I have extracted plant phenolics in aqueous methanol and want to prepare solution for gc-ms analysis to quantify phenolics. Help me in prep solution?

I want to quantify phenolics present in my aq. methanol extract using gc ms. But i want to make the extaract volatile so that it could separate out the phenolics. Please guide me to prepare my...

17 April 2018 6,145 4 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How long I can store plasma in -80 oC before doing Growth differentiation factor-15 assay ? , Is it different when I stored plasma at -20 or -70 ?

I want to do GDF-15 assay on multidrug resistant tuberculosis patient

29 December 2020 6,764 1 View

What are the invivo screening methods for anti TB drug?

what are the invivo screening methods for anti TB drug?

25 December 2020 722 1 View

Iam looking for articles on mycobacterium tuberculosis?

Hellow my fellows Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project...

02 December 2020 2,123 3 View

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View

Removing carbon sources from 7H9 storage media - Mycobacterium tuberculosis?

I am attempting to grow Mycobacterium tuberculosis in a minimal medium, with a single carbon source (cholesterol). However, I am inoculating strains from stocks with 7H9 storage media which...

03 March 2020 2,560 2 View

Is the concept of Tuberculosis Medication really well known to lung experts in India?

Is the concept of Tuberculosis Medication really well known to lung experts in India? Do they not mess with the patient's lives by prescribing heavy doses of multidrug therapy? Is it possible to...

27 February 2020 9,342 1 View

How can we liquify sputum sample to aim efficient M.tb DNA extraction from sputum sample? Can we extract Mtb DNA from sputum treated with NaOH-NALC?

I am currently facing difficulty in the DNA extraction because of the viscosity of the sputum samples. I am using the Chelex based extraction method which does yield in DNA but it is inefficient...

11 December 2019 3,153 1 View