Hopw to evaluate test compound against m. tuberculosis?

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How to calculate theoretical Ki from the docking results?

I have used Schrödinger (Glide) for docking

13 July 2024 3,003 1 View

What is the optimum molecular weight cut-off (MWCO) for concentrating exosomes in PBS?

Hi, I have isolated exosomes from mouse epididymis using SEC in 300ul eluate (PBS) volume. I intend to concentrate the sample in order to increase the protein concentration (for LC-MS/MS) but I am...

29 August 2023 9,013 0 View

Projected DOS using fully relativistic Pseudopotential in QE?

Hello I have a query regarding calculation of Projected DOS, using Quantum espresso (QE). We are studying about a oxide structure, and as it is spin polarized calculation we are using fully...

05 March 2023 8,180 0 View

DFT (Quantum Espresso): High negative frequencies in 1x1x1 Ni3Mo(001) with vacuum 20A ?

Hello!!! I am a novice in quantum espresso, so I need your help in this regard. We are currently working on the 1x1x1 Ni3Mo (001) surface, to find out the change in the Gibb's free energy when...

25 January 2023 7,794 2 View

Negative frequency in .phdos file calculated using ph.x on (1x1x1) of Pt(111))?

Hello !! I am a novice in Quantum Espresso. Please help me out in the following problem: We are trying to optimise Pt(111) surface , it is important to note that even though the attached files...

15 December 2022 6,786 5 View

Somebody kindly send template of World Journal of Engineering ?

Somebody kindly send template of World Journal of Engineering.

13 December 2022 4,752 4 View

How to convert 200x magnification to size ?

I want to convert scale in a microscopy image 200x to micrometer scale

21 July 2022 9,730 1 View

How to do quantification analysis of an IHC image of cardiac tissue using image J software or any other open source software ?

We want to quantify an IHC image for specific expression of proinflammatory cytokines using image J or any other academic software

25 May 2022 7,265 1 View

Control in microstructure with growth inhibitor?

Dear researchers, I wish to understand the role of grain growth inhibitor in controlling the microstructure of the ceramic pellet. How it controls the growth? Also, I wish to know the grain...

15 January 2021 4,196 3 View

What are the invivo screening methods for anti TB drug?

what are the invivo screening methods for anti TB drug?

25 December 2020 901 1 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

30 June 2024 6,502 5 View

How do i use researchgate to cunduct literature review ?

I am a first-time researcher in the field of nursing and i would like to conduct research about knowledge and attitude towards prevention and spread of tuberculosis.

11 June 2024 3,821 2 View

The gap in diagnosing intra-thoracic tuberculosis in children is not closed despite the advanncement in microbiology fields?

In 2024, childhood tuberculosis will still be a significant global public health issue, especially in underdeveloped nations (LMICS). It is true that the underestimation of the disease's extent...

31 May 2024 5,221 0 View

Does anyone face this problem with a plasmid prepared from Midi-prep culture?

Hello, I have propagated a plasmid form a single colony (size around 15 kb) using small scale culture (2 ml of TB with selected Antibiotic) and purified it using Mini-prep kits ( FastGene Plasmid...

14 May 2024 6,123 2 View

Is there any method to do TDM for TB drug Ethambutol in human plasma by UHPLC with PDA detector?

We are standardizing chromatographic conditions for first-line TB drugs in our newly procured UHPLC system. For Ethambutol, we are not able to get dedicated chromatogram. Can anyone suggest any...

24 April 2024 9,859 0 View

Recently we are develping methods for Therapeutic drug monitoring of TB drugs, Need to know the required calculation for final estimation of drugs?

Hello, everyone. This is Dr Prerna, SR Biochemistry, NITRD, New Delhi. We are developing methods for Therapeutic drug monitoring of first line TB drugs in our laboratory. After utilizing suitable...

17 April 2024 9,598 0 View

Any studies/PhD thesis that has taken pan country as the sample and used FGD for studying a public health problem?

The pan-country study could have further divisions/categorization based on different criteria. Any recommendations would be greatly appreciated. Thank you!

27 March 2024 991 1 View

Are there any expert in dendritics cells derivated from somebody blood monocytes?

I am interested in the study of dendritic cells response under infection with mutant strain candidate to vaccine against TB. But I am begginer in the work with dendritic cells, somebody can help...

20 March 2024 1,587 7 View

Regarding resazurin assay?

I am doing a resazurin assay to determine the MIC of plant extracts against Mycobacterium tuberculosis. The problem is the MIC is coming at a very high value. I read in one paper that even if...

01 January 2024 7,916 2 View

Can someone explain mathmatically how e-1 is 0.37? This is Riley-Wells equation in TB transmission?

If Iqpt/Q = 1 and the number of airborne quanta inhaled by susceptibles equaled the number of susceptibles, some susceptibles would escape infection and others would inhale more than one quantum....

24 December 2023 1,144 0 View