HNB detects Mg2+ released after addition of dNTPs to the growing DNA chain. Mg2+ is therefore released by both Phi29 and Bst polymerase, so wondering if there is a possibility of detection.
It is a fair assumption Prasanna. I am presuming that you have Phi29 at your disposal and that is why you ask this question. Primers are fairly cheap now a days, so it should not hurt to try.
Yes Ivan I did. There is a color change but it is difficult to read it as it is very very slow. Requires about 5 Painful hours for something to happen.