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Questions related from Prasanna Joglekar
I have purified PCR product using PCR purification KIT. I did a qubit assay to quantify the DNA and yield in the range of 90-110 ng/ul. I tried to dilute it the samples to 13ng/ul - 15ul range...
03 March 2016 9,395 3 View
Need to get sequences of many plasmids(Sanger), but the sequencing reaction doesn't work. Need a reliable non-kit based method to do cleanup before sequencing
11 November 2015 4,820 2 View
HNB detects Mg2+ released after addition of dNTPs to the growing DNA chain. Mg2+ is therefore released by both Phi29 and Bst polymerase, so wondering if there is a possibility of detection.
08 August 2013 3,196 4 View
