I've been using the HiPerFect reagent from Qiagen to transfect HeLa cells with siRNA. I've optimized the transfection protocol using the number of cells suggested by the reagent guidelines, and it has worked pretty well. But when I've tested the same protocol of transfection but with a lower number of HeLa cells, almost all of my cells have died. Does anyone knows if this reagent does not support a small number of cells for transfection? Or maybe an alternative protocol? Thanks!
Hi. Protocols given for transfection reagents are usually suggested proper amount of transfection mix for each type of TC vessels. Follow the suggestions.
hi
You better ask the Technical Assistance of QIAgene. Combined kit is patent for Company. But because of the expected arrival of a large number of siRNA in Cell could be induce apoptosis in cells.
good luck
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