Hi all,

Have you ever experienced that more mcrA-associated taxa are unassigned and uncultured in your project? Is it a normal phenomenon with this gene? In my analysis, many taxa are unassigned and uncultured so I have long been looking for evidence and reasons why this happened and also can I stick to the low known methanogenic taxa?

By the way, I have generated the mcrA database from NCBI website.

I would appreciate share your experience and guidance with me.

Thanks