17 Questions 44 Answers 0 Followers
Questions related from Mehrdad Rabiei
Dear friends, I need to know about melting curve in qPCR (Syber Green) cycle conditions. In the following paper, the context indicates melting curve from 72 C to 95 C for 15 min. Reagent and...
01 January 1970 6,416 3 View
Dear researchers, As you know, it might be some ambiguity nucleotides in primer oligos appeared. For example, in my case, which I took the forward and reverse primers from a paper, there are some...
01 January 1970 2,009 2 View
I could not find the PCR amplicon length by a primer set mentioned in a paper. I googled and followed the references, but not worked. Have you got any suggestion?
01 January 1970 3,218 5 View
I have two treatment groups of sample plus a group control. Each treatment and control have own three replicates. I am using Qiime2 for data analysis. I am in dilemma to the following ways below....
01 January 1970 4,874 3 View
I was trying to find alpha, beta and gamma diversities in the Brock biology of microorganisms book, but there is no such info in. Similarly, I could not find evenness and richness phrases. I have...
01 January 1970 5,700 7 View
Could you please tell me for what reasons we should use a cloned DNA segement as a standard to find out how much a certain gene expressed? If you need an example, take a look the Quantitative PCR...
01 January 1970 5,160 4 View
In sea salt, there are variety elements. How possible to prepare a molar of sea salt!? As you know, sea salt is primarily made of NaCl, around 85%. Is preparation of one molar sea salt as same as...
01 January 1970 5,512 1 View
Dear Friends, I am looking for a MiSeq Sequencing Center in Germany. If you know one, please share its name and address with me. *It is necessary to sequence my smaples by 2 × 300 bp platform. I...
01 January 1970 1,813 4 View
After taxonomy assignment, I received some unassigned taxa. I balsted the sequences an I understood they are uncultured bacterium clone. Now, I have three questions: 1. why they have nor got...
01 January 1970 9,207 3 View
I faced with a problem in Qiime2 when I was demultiplexing my data. The error is below: There was an issue with loading the file Barcodemetadate.txt as metadata: Metadata file path doesn’t...
01 January 1970 7,521 4 View
Hello dear friends, I need to know how to import data into STAMP software? The tutorial is incomplete. and should I import sequence counts obtained from taxonomy bar plot file or what? Please...
01 January 1970 3,104 0 View
How can I solve the problem? I used some ways but got the same error below! Thanks Plugin error from composition: All values in `grouping` are unique. This method cannot operate on a grouping...
01 January 1970 959 3 View
Hi, Merging two reads (R1 and R2) gave lower overlapping. I had to do microbial taxonomy assignment with single-end (R1). Is it an imperfect analysis? Is the result reliable? And what is your...
01 January 1970 2,409 0 View
Dear friends, I need the exact explanation of these terms: -cut-off value -lineage -syntax -proxy -k-mer Also, please tell me differences between aligned and unaligned sequences in databases....
01 January 1970 7,801 1 View
Dear Friends, I am going to analyze the mcrA gene dataset. The trimming, truncating, chimera removing steps have been already done. Now, I need a database to do that. Please help me how can I find...
01 January 1970 6,815 0 View
Hi friends, I am working on mcrA gene. To do this, I need a database, reference taxonomy, and the relevant stuff to draw a taxonomy bar plot. SILVA and Greengenes either have data for bacteria...
01 January 1970 4,757 7 View
Hi all, Have you ever experienced that more mcrA-associated taxa are unassigned and uncultured in your project? Is it a normal phenomenon with this gene? In my analysis, many taxa are unassigned...
01 January 1970 7,687 0 View
