Hi
We recently bought Nikon eclipse Ti2 inverted fluorescent microscope.
https://www.nikoninstruments.com/Products/Inverted-Microscopes/Eclipse-Ti2
It claims that it can get very high-quality images. In bright field it is OK (not superb!) but when switches in phase contrast, the quality of images are not good at all. The technician from Nikon just introduced the image processing capability of the system.
The instruction of the device is also full of explaining unnecessary data and is not user-friendly. Does anyone have any suggestion to get better quality images using this system?
Well, if the microscope is not damaged then the most likely culprit is that it is not calibrated correctly. Have you gone through the entire process of aligning the optical path? Are your phase plate and annulus properly matched? Is your Kohler illumination aligned? Are your samples sitting flat on the stage? Is your camera in the correct alignment in the optical port?
- https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy
- https://www.microscopyu.com/tutorials/kohler
The microscope itself is professional grade. You should be able to take clear images in any mode so long as it is in working order.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Physical Phenomena
- Luminescence
- Fluorescence