Hi, I'm going to study the binding affinity of my protein with calcium ions. I'm looking for a buffer that can control the pH around 8.5 or more. I heard that Tris is not really good for ITC so I'm looking for another buffer that can control pH at 8.5 or more. May I have some advice on this? Thank you in advance.
Adam B Shapiro
Here is a handy buffer table:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-concentration-and-buffer-exchange/buffer-reference-center
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Adrian Velazquez-Campoy
In this review:
https://www.nist.gov/system/files/documents/srd/jpcrd615.pdf
you will find thermodynamic data on buffer ionization for many buffers, with relevant information for ITC (pKa and buffer ionization enthalpy).
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