Hi, My ClickIt reactions used to give me 3-4 orders of magnitude of separation in FACS. Now the positive and negative peaks are so close together that the results are hard to interpret. Any suggestions what might have caused this? Many thanks.
I'd love your help understanding this question. I'm confused about what causal claims the Cattell–Horn–Carroll (CHC) theory of intelligence makes. As you likely know, it defines a hierarchy of...
04 February 2023 1,681 1 View
I used to do EdU + ClickIt staining regularly. 2 months ago it stopped working. Specifically, in FACS, all of the cells are positive every time, despite my changing the cell type and all of the...
28 December 2021 5,013 0 View
hallow We bought tert-Butyl hydroperoxide at a concentration of 70%. How do i calculate the concentration to produce working concentrations in uM? thanks
19 December 2020 8,492 1 View
We performed an experiment on a number of blood donors. We took the blood from each donor and divided it into 9 equal volumes. Three of the blood samples from each individual were left as...
17 May 2020 9,522 5 View
I wanted to start a project to test drugs on CLL cells. at this point i dont want to use sample from hospitals; therefore, I would like to know which commercial leukemia cell lines should be the...
29 January 2016 9,710 3 View
no more deatail
24 January 2016 5,037 0 View
Do you use 0-6m protocol or maybe even 0-3m, 3-6m etc..? Did you change the protocol for different CT systems?
01 October 2015 8,823 2 View
Hi I am attempting to find clinical research papers to assist me with the causal nexus between small cell B cell follicular non-Hodgkin lymphoma and second hand tobacco smoke Thanks Lionel Greenberg
04 May 2015 1,736 0 View
We are looking for a cell based assay to test different drugs for treating depression, before going to the mice model. Any ideas? We would love to learn. Thanks in advance.
14 March 2015 9,694 1 View
I'm looking for databases of peptides i could compare to the results I got from NGS Any suggestions? thanks
17 December 2014 4,106 5 View
i am unable to interpret why its increases in start as shown in figure
11 August 2024 2,179 1 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
How can I interpret the data gathered without solving?
03 August 2024 9,054 3 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View