I'll thank you in advance for the help.
I am doing research for my thesis which is comparing the genetic variants (genes, SNPS, etc.) of 4 medical conditions. I have identified approximately 7,000-10,000 genetic variants that I would...
01 January 2020 10,150 2 View
Dear colleagues, Which is better - to sow a former A. tumefaciens infested land with corn/wheat or to leave it without any crops for a couple of years (2-3)? My colleagues are more inclined to...
28 August 2018 5,819 2 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...
05 August 2024 8,025 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...
18 July 2024 5,225 3 View
I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...
08 July 2024 9,201 2 View
The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...
01 July 2024 2,356 6 View
Image attached is at 20X. Cultured media for checking presence of bacteria and yeast and both tests were negative.
27 June 2024 7,473 4 View
Publier des documents scientifiques
26 June 2024 7,343 1 View
I'm a newbie to time-kill test, and tbh I've read every article about synergy between two or more drugs together but I'm still unable to grasp the nature and the standard protocol of a time-kill...
25 June 2024 7,392 0 View