09 January 2012 2 920 Report

Lipid and Cell-Wall Analysis in Bacterial Systematics

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Can you tell me what type of T test I should run?

Determination of vitamin d in egg yolks. The eggs are collected from farms with different husbandry conditions. Caged, free range and organic eggs. I have data collected today. Concentration of...

03 December 2023 1,998 1 View

In order to determine the vitamin D level in chicken eggs, what method should I use?

I will be analyzing the vitamin D content of chicken eggs. I have been advised to use Perkin Elmer Gas Chromatography as the most appropriate gas chromatograph for this experiment. What steps are...

12 October 2023 8,300 3 View

My housekeeping gene has higher CT values than the interested samples. How can I overcome this issue?

I have been doing some qPCRs on bacterial genomic DNA. I am using 16S as my "housekeeping gene". My sample of interest, however, yield a lower CT value. I am trying to figure out how to overcome...

20 April 2023 2,121 3 View

Is it possible to generate cell line from frozen tissue?

I want to preserve tissues from plaque in peyronie's disease. Then I will do primary culture. Is this possible, does it preserve cell viability? I store the tissues in the RNA save solution. How...

15 August 2022 4,054 1 View

Do you have a problem with adding a new publication?

Dear official, I have a problem with adding a new publication. The 'Upload' button is not active when I add a new publication. Is there a problem with the system? I will be glad if you can help...

27 August 2021 7,798 6 View

How can I isolate RNA and synthesis cDNA from mouse serum samples?

I obtained mouse serum samples. My initial plan is to check some proteins using ELISA. However, plans have changed and now I need to check some gene expressions. My questions are: 1) Can I...

08 June 2021 823 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 7,202 3 View

I want to ask how we can calculate large strains for a hyperelastic brick element in abaqus ?

if we choose GeoNLA for hyperelastik materials for ABAQUS, i think it calculates small strain teory. But known that that materials (rubber lilke, silicone exc.) have to be calculated large strain...

24 October 2019 555 0 View

Is there any way to determine in vivo whether a drug's effect is presynaptic or postsynaptic?

In animal studies, when you give a drug that binds both presynaptic and postsynaptic receptors (e.g. alpha agonist), how to determine whether the drug binds to pre or postsynaptic receptor in a...

21 July 2019 9,818 1 View

Could you please recommend a good emotion regulation scale for 4 to 6 years old children?

I prefer a scale an adult would fill up about a child. Bu also I would like to learn more about observation tools. Thanks

24 March 2018 7,870 3 View

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Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

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Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

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First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

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How to determine method detection limit in an analytical method?

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Do we put respondents data in research methods or findings section of a qualitative study?

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