Hi, I am currently optimizing FACS analysis. The levels of CD19 in mouse bone marrow is varying between 50-90%. I have included FC blocker and live/dead viability stain. I would like to know, the range of CD19 levels in bone marrow and your comments. Thank you.
Hello,
You could run a positive control experiment (B-cells overexpressing CD19 for example) and see what is the variation in this case. You could use a B cell line and use lentivirus for stable expression of CD19. If there is a variation then this would mean the variation is caused by the device. If it is stable then the difference is caused by biological origins. Basically its hard to answer without a positive control. All the best
Onur
How do you do the compensation?For animal FACS is better to do compensation everytime you do the FACS