Hi, Im trying to decide on the dilatation factor for complement pathway assay for samples prepared from tissue?

13 April 2022 1 1K Report

When using the neat samples the concentration of C3b could not be extrapolated beyond the highest concentration on the STD curve!

I would appreciate your thoughts if you have experience in this..

Tahmina Begum

Hi Tanweer

You can find some help from:

https://www.hemocytometer.org/dilution-factor-calculator/

Thanks

Badges
Science topic

More Tanweer Beleil's questions See All

Can anyone suggest impact factor journal with fast publication. Related to Health sciences, nutrition, health informatics, public health, medicine?

I need fast publication

11 September 2021 7,081 3 View

How to write review of a Research Paper? What ethics should a referee follow ?

What are the basic ethics to be followed to write a review report of Research Paper?

24 June 2021 1,200 10 View

Which dataset to use for motion artifact removal application using photoplethysomograph signal PPG?

Is it better to use Signal Processing Cup 2015 or PhysioNet dataset for getting photoplethysomograph signal for motion artifact removal application ? SPcup data provides 2-channel ppg with...

08 October 2016 5,728 0 View

Can anyone suggest me good book for learning OMNeT++.

09 September 2016 8,861 14 View

How do I run the molecular dynamics of beta-cyclodextrin and p-nitrophenol on abalone?

I am working on abalone software and I tried running the MD of cyclodextrin and nitrophenol.As soon as I start the MD,nitrophenol gets deformed.How to solve this problem?

02 May 2015 2,657 8 View

What are the steps behind formation of imagination? Are they different from thoughts?

How imagination is form... How is it helpful in learning mechanism... How can we estimate one action before taking ours.... How can we implement imagination in machines.....

01 March 2013 3,548 10 View

Can we achieve creative problem solving using imagination?

We mostly simulate our problems before action selection. Can we apply this in machine to achieve human like problem solving?

12 November 2012 5,955 12 View

How can one simulate a PPG signal using MATLAB ?

I need to generate/simulate a Photoplethysomography (PPG) signal in MATLAB as real time hardware acquisition is not possible at this time. Thanks in Advance

01 January 1970 5,860 5 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Is space-time dilation conceptually equivalent to space-time expansion?

Relativistic space-time is described as a four-dimensional continuum comprising three dimensions of space and one dimension of time. In this framework, space and time are interwoven, forming an...

29 July 2024 5,365 2 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View

What is the best method for removing paraffin from plant samples prepared for microtome?

...

24 July 2024 3,087 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

How many samples size should I select to compare both groups?

I want to study the differences between two groups: the treatment group and the comparison group. The total population consists of 60000 women in the treatment group, distributed across different...

21 July 2024 669 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View