Hi, I want to stain either a OH group or a Methyl group in lysine to separate them on a column chromatogram. What dyes can I use?

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Abid Aziz Ajaz

Ninhydrin is still one of the best reagent to TLC-Identify the amino acids . So by this technique , establish first the aminoacid separation pattern & then use little more concentrated solvent system than the TLC for the column chromatography to separate this aminoacid from their mixtures. I think this should help you for you research work. Dr Abid Aziz Ajaz .

Grigor B Bantchev

Separate them from what? Other aminoacids? Strictly speaking, lysine and Cbz-lysine have neither methyl, nor hydroxyl groups. Do you mean lysine-rich peptides? Methyl groups are very hard to react.

https://en.wikipedia.org/wiki/Lysine

https://en.wikipedia.org/wiki/Carboxybenzyl