The brain tissue its from a lizard. We want to see de serotonin mark in the pineal complex.
There are multiple options for removing autofluorescence from tissue, depending on the resources available to you. A traditional method is to use lipophylic dyes that are able to sequester the intrinsic fluorescence. This methods is relatively inexpensive and easy to use, but does come with some downsides in that it can also sequester your desired signal, and it can compromise the integrity of the tissue itself. Another option is to image the tissue on a microscope that has the ability to spectrally-characterize both the autofluorescence and the signal of interest, and then mathematically categorize each pixel in the image as one or the other. This process is called spectral imaging and linear unmixing. Here is a link to a paper from our laboratory that compares the two methods and explains how they work in better detail. Article An Alternative to Dye-Based Approaches to Remove Background ...
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