Hi, I am trying to prepare Pst DC300 Comp cells, but i dont think i am close to success. any suggestions?

01 January 1970 0 3K Report

Hi, I have tried both chemical and electro-competent cell methods to prepare the comp cells but have failed. For Chemical method, I have used 0.1M MgCl2/CaCl2 and 15% glycerol method. And for electro-competent cells, I have used 300mM sucrose method. I am attaching the protocols for better understanding. Please help me with this.

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