Arpita Das

3 Questions 3 Answers 0 Followers

Questions related from Arpita Das

Why is my PCR not working from genomic DNA?

I am trying to check the homozygous mutant for Arabidopsis (seeds ordered from ABRC). I have done genomic DNA extraction, using Edward's method and checked the it in agarose, the genomic DNA is...

29 March 2024 755 3 View

I am trying to express and purify a plant protein in arctic cells(E.coli), but its not binding with Ni+ beads. Can anyone suggest how to purify it?

my protein His-Sumo-insert (Theoretical pI: 5.84), is a plant protein, and it's getting expressed in ecoli, but everything was going into pellet fraction. i have tried changing cells (BL21,...

14 March 2024 2,082 3 View

Why is my Luciferase assay for understanding the refolding efficiency of Hsp70 chaperone system [invitro] coming in a zigzac fashion w.r.t time??

I am trying to standardize luciferase assay so I am using 1.6uM DnaK, 320nM dnaJ, and 800nM GrpE, to refold 10uM of thermally[30 degree for 10 min] denatured luciferase[final conc 80nM in the...

10 February 2022 1,714 2 View