Arpita Das

4 Questions 3 Answers 0 Followers

Questions related from Arpita Das

Why is my PCR not working from genomic DNA?

I am trying to check the homozygous mutant for Arabidopsis (seeds ordered from ABRC). I have done genomic DNA extraction, using Edward's method and checked the it in agarose, the genomic DNA is...

29 March 2024 914 3 View

I am trying to express and purify a plant protein in arctic cells(E.coli), but its not binding with Ni+ beads. Can anyone suggest how to purify it?

my protein His-Sumo-insert (Theoretical pI: 5.84), is a plant protein, and it's getting expressed in ecoli, but everything was going into pellet fraction. i have tried changing cells (BL21,...

14 March 2024 2,180 3 View

Why is my Luciferase assay for understanding the refolding efficiency of Hsp70 chaperone system [invitro] coming in a zigzac fashion w.r.t time??

I am trying to standardize luciferase assay so I am using 1.6uM DnaK, 320nM dnaJ, and 800nM GrpE, to refold 10uM of thermally[30 degree for 10 min] denatured luciferase[final conc 80nM in the...

10 February 2022 1,770 2 View

Hi, I am trying to prepare Pst DC300 Comp cells, but i dont think i am close to success. any suggestions?

Hi, I have tried both chemical and electro-competent cell methods to prepare the comp cells but have failed. For Chemical method, I have used 0.1M MgCl2/CaCl2 and 15% glycerol method. And for...

01 January 1970 3,046 0 View