Hi, I am isolating exosomes from plasma by size exclusion chromatography and whenever I try to analyze them by mass spec I don't get any exosomal markers at all. I need hints about what is the starting material I should use (I went up to 5 ml) and whether or not there's a way to get rid of all the albumin and immunoglobulins contaminating my samples.
Tim Schelfhorst
You can try to deplete the sample before or after the SEC using depletion columns, but my guess would be that it is not going to work.
LC-MS/MS and plasma (derived EV's) generally don't go well. Protein like albumin, immunoglobulins, and more of those will always dominate your sample.
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