Tim Schelfhorst

5 Questions 26 Answers 0 Followers

Questions related from Tim Schelfhorst

Isolating microvesicles from plasma using Size Exclusion Chromatography(SEC) doesn't show results on western blot, are there enough vesicles?

Hi, I am having trouble isolating microvesicles from 1ml of healthy patient plasma using size exclusion chromatography. I am trying to validate this method using western blot with CD63 and CD81....

27 August 2015 6,808 5 View

I am looking for a stringent wash buffer that does not lysate exosomes?

Hi, I have isolated exosomes from plasma, the exosomes are in some sort of protein aggregate. Is there anyway to wash away other plasma proteins (albumins, fibrinogens etc.) without lysing the...

01 December 2014 8,936 4 View

Can anyone help with transforming pCS1966 into E. Coli?

We have now attempted several times to transform our pCS1966 plasmid 5.4kb(approx.), containing an insert. The pCS1966 contains a erythromycin resistance and an orotate transporter. We have tried...

09 May 2014 7,669 14 View

Can someone recommend a 25mm opening culture vessel which is small?

I'm looking for a culture vessel of some kind, with a opening of at least 25mm, and dimensions closest to 60mmx30mmx5mm. Preferably not round but flat. The only option I now have is a T150 flask...

29 October 2013 414 8 View

Does anyone have an idea, how long it will take for a single mcf7 cell to grow into a clump, or double for that matter?

Does anyone have an idea, how long it will take for a single mcf7 cell to grow into a clump, or double for that matter? I want to culture from single cells.

10 October 2013 1,668 12 View