Tim Schelfhorst

4 Questions 21 Answers 0 Followers

Questions related from Tim Schelfhorst

Isolating microvesicles from plasma using Size Exclusion Chromatography(SEC) doesn't show results on western blot, are there enough vesicles?

Hi, I am having trouble isolating microvesicles from 1ml of healthy patient plasma using size exclusion chromatography. I am trying to validate this method using western blot with CD63 and CD81....

27 August 2015 6,765 5 View

I am looking for a stringent wash buffer that does not lysate exosomes?

Hi, I have isolated exosomes from plasma, the exosomes are in some sort of protein aggregate. Is there anyway to wash away other plasma proteins (albumins, fibrinogens etc.) without lysing the...

01 December 2014 8,893 4 View

Can someone recommend a 25mm opening culture vessel which is small?

I'm looking for a culture vessel of some kind, with a opening of at least 25mm, and dimensions closest to 60mmx30mmx5mm. Preferably not round but flat. The only option I now have is a T150 flask...

29 October 2013 373 8 View

Does anyone have an idea, how long it will take for a single mcf7 cell to grow into a clump, or double for that matter?

Does anyone have an idea, how long it will take for a single mcf7 cell to grow into a clump, or double for that matter? I want to culture from single cells.

10 October 2013 1,598 12 View