Hi, help please, how to cancel the authorship of the article Civilizational Phenomenon of Narcissism: Vectors of Objectification in a Paradigm...?

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SPSS: A scale with multiple items (7-point Likert) across 4 test conditions. How can I correctly summarize it?

Hello, I am currently writing my master thesis where I have a 2x2 factorial design with one downstream variable (2 independent and 2 dependent variables). I need to conduct a multivariate...

11 February 2021 6,014 4 View

Where I can order fungal ITS1 and ITS4 primers?

I would greatly appreciate for help in the suggestion of company for ITS1 and IT4 primers purchasing in Europe. Thank you.

12 September 2017 469 3 View

Could the gene silencing be avoided when it is expressed from the episomal plasmid?

In the case of yeast transformants

14 March 2016 9,434 1 View

Does anyone know some kits for express diagnostics basic soil characteristics?

Dear colleagues, I need to estimate the basic soil characteristics - total carbon, nitrogen, potassium content. I also need the content of Fe and halogens (F, Cl, maybe I). The point is that I can...

12 February 2015 4,874 9 View

Has anyone used Mesquite for combining molecular and morphological data? Do you know a algorithm for doing that?

Phylogenetic analysis (combining molecular and morphological data).

10 August 2014 4,406 7 View

Could you please suggest a technique of a plant root preparation for XRF confocal imaging?

Zou Finrock and Kelly Summers have recommended me to contact you. We are using 8-day old Arabidopsis plants to see of the effects of toxic metals, in the root tips, in particular. They are really...

01 January 1970 5,600 3 View

What do you think about the status of ownership in the charing economy?

We understand that in sharing economy, access to usage will be important. How will the attitude to ownership and disposal of property change? Do you want be the owners?

01 January 1970 546 8 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

How to best engender critical thinking in K-12 education?

From my observations, there appear to be three principal avenues to engender critical thinking in education K-12: debate; mathematics, and science. For some students, all three avenues are...

04 February 2021 9,605 7 View

James R Knaub

If you go to the upper right corner of your screen, in most cases I think, there are three dots (on my phone anyway). If you click on that and scroll down the menu, you should find "help & feedback." Sometimes "help" is at the bottom of the screen. You can then ask ResearchGate staff to help you.