Hello, ı want to produce a thick layer of SU8-3050 -more than 100 micrometers-. Instead of a double layer, can ı perform the spin coating under 1000 rpm, does it form a homogenous layer?
I am working with yeast saccharomyces cerevisiae -BY4741- strain. I tagged the Atg8 with GFP in this background and whenever I am performing western blot I get a free GFP signal for my WT which...
15 November 2020 8,556 3 View
in vitro mice CD8 cell activation. These cells are extracted from B6 mice spleen by magnetic separation.
01 November 2020 3,276 2 View
I have 2 drugs (PTC-209 and GSK343) that are soluble in DMSO (I already made the stock solution, dissolved to a clear solution) and I have to inject them IP to mice for a 2 week treatment course....
17 September 2020 9,107 3 View
Hello, I'm trying to model my dataset with decision trees in Python. I have 15 categorical and 8 numerical attributes. Since I can't introduce the strings to the classifier, I applied one-hot...
24 July 2020 5,787 3 View
The reference electrod I use is saturated calomel electrode (3M KCl as the filling solution). I got overload problem while measuring the EIS, so then I checked the impedance of the electrod and...
27 May 2020 7,557 12 View
In 1993, Lent et al. introduced one of the most promising and emerging computational paradigm known as Quantum-dot Cellular Automaton (QCA) computing. Now, it is almost 30 years have been passed,...
06 May 2020 4,955 7 View
During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?
04 May 2020 4,700 11 View
Hi all, I have a couple of questions for PMF calculations in Gromacs. I placed a nanoparticle (NP) in the water region approximately 1nm away from the membrane and by using Gromacs pull code, I...
21 April 2020 3,827 4 View
I am looking to communicate with a personnel who deals with beginner-level review articles, and want to learn how to write good reviews and the rules and regulations to follow.
06 March 2020 6,068 3 View
We observe some cracks among the nanotubes which are formed on the surface of the Ti alloy after anodic oxdation. What could be the cause of this problem?
13 January 2020 3,086 9 View
Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...
01 March 2021 8,220 2 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View
Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax
28 February 2021 6,685 2 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...
27 February 2021 1,829 3 View
It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?
26 February 2021 1,760 8 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...
25 February 2021 7,994 3 View
I tried coating Fe3O4@ SiO2 by using APTES in 5% water-ethanol but it is not coated. Kindly suggest a solution for it. Thanks
24 February 2021 5,992 4 View