Hello, ı want to produce a thick layer of SU8-3050 -more than 100 micrometers-. Instead of a double layer, can ı perform the spin coating under 1000 rpm, does it form a homogenous layer?
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,672 6 View
I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...
07 July 2024 8,670 6 View
I'm working with HEI-OC1 cells of the inner ear. Recently I'm seeing these round particles in the flask (picture attached), but cells' morphology and growth seems normal. However, there are...
26 June 2024 8,717 7 View
Hello, I aim to compare the Length of Stay (LOS) in hours b/n two independent groups, with sample sizes of 300 and 310. The LOS data does not follow a normal distribution and shows right-skewed...
25 June 2024 1,842 7 View
Hi , in my study I will compare the effectiveness of Al generated feedback and traditional feedback in writing lesson.It will be a quasi-experimental design,but l have 3 classes not two.What...
25 June 2024 6,244 3 View
Hi everyone, I was wondering how to get the correlation between one dependent variable and independent variable, Pearson correlation is not an option as there is a follow up visit for each...
24 June 2024 8,104 6 View
Hi everyone, we know that the analysis of pilot studies should be based on descriptive statistics and ideally not involve any inferential statistics (i.e., P values). Based on this can I use Roc...
24 June 2024 4,402 7 View
The Analyse for Pilot study should be based on the descriptive stat and ideally not involved inferential stat. what about exploratory study? can I do inferential stat like cox regression or...
17 June 2024 5,468 3 View
Hi everyone, I've created a p-chart to monitor a binary variable on a quarterly basis. But I noticed a p-chart requires at least 20 data points. So, I've shifted to monthly reporting. with this...
06 June 2024 4,849 0 View
Hi everyone, what assumption will be used if the assumption of one-way Anova are unmet? I think Kruskal Wallis-test, am I right? What about two-way Anova?
03 June 2024 2,220 3 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 940 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,297 0 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,524 4 View
I am currently using CASTEP version 2020. When I want to run optical properties calculations with spin-orbit coupling(SOC), I encounter the following error in a pop-up window before the job gets...
02 August 2024 2,890 0 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,291 3 View
In the context of Electron Spin Resonance (ESR) spectroscopy, under what conditions is an absorption spectrum considered to be a single absorption spectrum?
25 July 2024 2,325 1 View
I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose
25 July 2024 438 3 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,073 2 View
We are trying to coat some cathode materials on Al-foil ( ex - LiFePO4 , S@CNT, etc). When we coated the Al foil with the cathode (ex - S@CNT) material , the cycling result was annoying. But the...
09 July 2024 5,122 4 View
I tried to make slurry for NCM cathode but there was problem in coating process. The detail of my experiment is following; The ratio between active material(NCM811), conductive additives(Super-P)...
08 July 2024 7,327 4 View